Fig. 4

Flow diagram of the current analysis pipeline to identify non-canonical motifs. The pipeline combines the standard practices of HT-SELEX data modeling [29] with an additional set of conservative filtering criteria to eliminate experimental and statistical artifacts (shown in red). These criteria include selecting L-mers (sequences of length L) with a minimum count and a minimum di-nucleotide entropy, followed by selecting L-mers that have a minimum enrichment, positive enrichment over successive rounds, and alone occurrence (i.e., occurrence independent of the canonical motifs)