Fig. 4From: MDMX is essential for the regulation of p53 protein levels in the absence of a functional MDM2 C-terminal tailNuclear sequestration reduces MDMX activity to suppress p53 protein expression. A MDM2487/487 MEF cells were treated with empty vector (EV), MDMX or NLS-tagged MDMX lentiviral constructs for 48 h prior to immunostaining using an anti-MDMX antibody, and DAPI nuclear staining. B MDM2487/487 MEFs were treated with empty vector (EV), MDMX, or NLS-tagged MDMX lentiviral constructs for 48 h prior to lysis and immunoblottingBack to article page