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Fig. 8 | BMC Molecular and Cell Biology

Fig. 8

From: Intercellular transfer of mitochondrial DNA carrying metastasis-enhancing pathogenic mutations from high- to low-metastatic tumor cells and stromal cells via extracellular vesicles

Fig. 8

S-EV-mediated mtDNA transfer to ρ0P29 cells. (a) Schematic drawing of the procedure of S-EV-mediated transfer of mtDNA. S-EVs isolated from the conditioned media of P29 and A11 cells were incubated with ρ0P29 cells in the presence or absence of EV-Entry reagent for 2 days. The cells were detached from the dishes by trypsinization and washed extensively with PBS. (b) PCR analysis of mtDNA transfer to ρ0P29 cells via S-EVs. S-EVs-exposed ρ0P29 cell DNAs were isolated and subjected to PCR amplification of the ND1 and ACTB genes. The PCR primers for ACTB gave rise to two bands. As inputs, one-twentieth of the amount of S-EVs added to ρ0P29 cells was also subjected to PCR analysis

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