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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing

Fig. 2

Isolation of single cells from ossifying posterior longitudinal ligament and scRNA-seq. A. Ossification of posterior longitudinal ligament (OPLL) was shown on the three-dimensional reconstruction of computerized tomography (CT) image (red circle). B. Gross appearance of the ossifying posterior longitudinal ligament harvested during surgery. C. Gross appearance of the cell suspension and residuals after 3–5 cycles of enzymatic digestion. D. Stereo microscope images showed the residuals. The red box enclosed some white soft viscous residuals magnified in the image labeled 1. And the yellow box marked part of the bone fragments magnified in the image labeled 2. E. Total cell numbers were counted automatically and manually, respectively. F. The isolated cell diameter distribution histogram. G. The live/dead immunofluorescence staining and quantitative analysis. H. Bioanalyzer trace of the scRNA-seq library showed the size distribution between 300 and 600 bp (blue arrow). I. The barcode-rank plot displayed gene expression counts after sequencing

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