Fig. 3
From: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing
Rapid isolation of single cells from knee articular cartilage with osteoarthritis (OA) and the quality control. A. OA was characterized on T2-weighted imaging (T2-WI). The affected region was circled in red. B. The isolated cell diameter distribution histogram. C. The live/dead immunofluorescence staining and quantitative analysis. White arrowheads highlighted the cell aggregates, which showed Hoechst 33342+, Calcein+ and PI−