Fig. 4
From: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing
Prolonged isolation of single cells from knee articular cartilage with OA and the quality control. A. The total cell numbers counted automatically in both rapid and prolonged enzymatic treatment groups. Prolonged and continuous digestion would significantly increase cell yield (P < 0.01). B. The isolated cell diameter distribution histogram. C. The live/dead immunofluorescence staining and quantitative analysis. Yellow arrowheads highlighted the cell aggregates, which showed Hoechst 33342+, Calcein− and PI+