From: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing
Reference | Classification of IVD | Species | Treatment | Dead cell removal | Profiling cellular types |
---|---|---|---|---|---|
Gan Y, et al. [10] | Pfirrmann grade I | Human | TrypLE Express for 30 min, 0.2% pronase (Sigma-Aldrich, USA) for 60 min, and 0.012% collagenase II (Sigma-Aldrich, USA) for 2 ~ 4 h, sequentially | Yes | Chondrocyte, Notochord, Nucleus pulposus progenitor cells, Stromal cell, Pericyte, Endothelial cell and blood cell |
Fernandes LM, et al. [11] | Thompson grade 1 or 2 | Human | Pronase for 1Â h and then collagenase P overnight | No Culture cells for scRNA-seq | Cultured nucleus pulposus cells |
Calió M, et al. [12] |  | Bovine | 0.19% Pronase for 1 h and then 260 IU/mg Collagenase II overnight | No | Nucleus pulposus cells, uncharacterized cell subset, monocyte derived dendritic cells |
Zhang Y, et al. [13] | Pfirrmann grade I-IV | Human | GEXSCOPETM Tissue Dissociation Solution for 15 min. (Included in the GEXSCOPE® Single Cell RNA library Kit) | No | Chondrocytes, macrophages and endothelial cells |
Ji Tu, et al. [14] | Pfirrmann grade II-V | Human | 500 U/mL collagenase I, 150 U/mL collagenase II, 50 U/mL collagenase IV, 0.1Â mg/ml hyaluronidase and 30 U/mL DNaseI for 95Â min | Yes | Neucleus pulposus cells, natural killer cells, monocytes, and T cells |