Fig. 4
Analysis of mRNA expression in iPSC and ESC-derived differentiated cells over time. a mRNA expression in iPSC-derived differentiated cells regarding the genes related to the joint interzone (Wnt16 and Wnt9a), superficial zone (Prg4, Erg, Tnc and Runx1) and fibroblast-like synovial cells (Cdh11, Col4a1, Vcam1, Icam1, Cd55 and Cd248). Data are expressed as the mean ± standard deviation of three technical replicates per n and n = 3 biological replicates. *P < 0.05; **P < 0.01; and ***P < 0.001. D0, day 0; D12, day 12; and D21, day 21. Embryonic stem cells (ESCs) were used for comparison. b mRNA expression in ESC-derived differentiated cells regarding the genes related to the joint interzone, superficial zone, and fibroblast-like synovial cells. Data are expressed as the mean ± standard deviation of three technical replicates per n and n = 3 biological replicates. *P < 0.05; **P < 0.01; and ***P < 0.001. D0, day 0; D12, day 12; and D21, day 21