Fig. 5

RNA-sequencing analysis of mRFP1-positive cells obtained by FACS. a Flow cytometry analysis of embryonic stem cell (ESC)-derived differentiated cells (control) and iPSC-derived mRFP1-positive cells (#2–9) on day 21. P4 gate of negative control was set to < 0.1%. Positive cell ratio of iPSC-derived mRFP1-positive cells was average 4.9% (maximum 9.3%) (n = 16 independent experiment). b Heatmap showing clustering analysis of RNA-sequencing data obtained from muscle (Musc), mesoderm (Meso), fibroblast-like synovial cells (FLS), iPSC-derived mRFP1-positive cells (#2–9), chondrocytes (Chon), macrophage-like synovial cells (MLS), and ESCs (ES). Myogenic (a) mesoderm (b), MLS (c), pluripotency (d), chondrogenic (e), interzone (f), FLS (g), and superficial zone (h) markers were assessed