Fig. 2

The effects of Nck1 on physiological properties of Jurkat T cell. a Cell characteristic under holotomographic microscope. Cells were left untreated or treated with anti-CD3 antibody (OKT3) alone for 5 min or pre-treated with AX-024 for 30 min followed by OKT3 stimulation for 5 min. Un-labelled cells were observed with holotomographic microscope. One representative image from three independent experiments was shown, n = 3. b cell size, c cell volume and d refractive index (RI) of wild-type and Nck1 knocked-out Jurkat T cells were identified using Tomostudio software. one representative of topographic images of 3D RI tomograms was shown. The colour bars represented the 3D-rendered RI distribution range (n = 1.345 − 1.500). Data are presented as the mean ± SD from three independent studies, n = 3.; ns = non-significant; **p < 0.01