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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Lack of Nck1 protein and Nck-CD3 interaction caused the increment of lipid content in Jurkat T cells

Fig. 4

The effects of Nck1 deficiency on lipid metabolite profile in Jurkat T cell. a Lipid metabolite profiles of Nck1-deficient cells. Cells were unstimulated or stimulated with anti-CD3 antibody (OKT3) alone for 5 min or pre-treated with AX-024 for 30 min followed by OKT3 stimulation for 5 min. Cells were processed for lipid metabolites analysis using Gas-Chromatography-Quadrupole Time of Flight mass spectrometer (GC-QTOF). Level of each of lipid metabolites was normalized by myristic acid and presented as bar graph. One representative image from three independent studies was depicted, n = 3. b Nck1 knock-out caused an increase of various lipid metabolites. Cells were treated and analysed as above. Selected of lipid metabolites were shown. Data were representative of three separated experiments, n = 3 (mean ± SD). ns = non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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