Fig. 4

ACOT7 suppresses apoptosis and ferroptosis. A siNC, siACOT7#1 and siACOT7#2 A549 cells were stained with PI and Annexin V and then subjected to flow cytometry analysis. A⁺P⁻, Annexin V⁺PI⁻; A⁺P⁺, Annexin V⁺PI⁺; A⁻P⁺, Annexin V⁻PI⁺. *p < 0.05. B H1299 cells transfected with Ctrl and ACOT7 overexpression lentivirus were stained with PI and Annexin V and then subjected to flow cytometry analysis. A⁺P⁻, Annexin V⁺PI⁻; A⁺P⁺, Annexin V⁺PI⁺; A⁻P⁺, Annexin V⁻PI⁺. *p < 0.05. C and D Lipid peroxidation was examined by using BODIPY 581/591C11 in H1299 cells transfected with Lenti-Ctrl and Lenti-ACOT7, and in A549 cells transfected with siNC, siACOT7#1 and siACOT7#2. *p < 0.05. **p < 0.01. E siNC, siACOT7#1 and siACOT7#2 A549 cells were treated with DMSO, ferrostatin-1 (Ferr-1, 2 uM), Z-VAD-FMK (Z-VAD, 8 ug/ml) and Ferr-1 + Z-VAD and cell viability was tested by trypan blue staining. *p < 0.05. **p < 0.01. n.s., not significant. F H1299 cells transfected with Ctrl and ACOT7 overexpression lentivirus were treated with DMSO, erastin (5 uM), apoptosis activator 2 (AA2, 4 uM) and erastin + AA2 and cell viability was tested by trypan blue staining. *p < 0.05. **p < 0.01