Fig. 5

Increased SFRP2 expression in EEECs and endometriosis tissues. A Western blot analysis of SFRP2 protein level in ectopic endometrium vs. NE(Normal endometrium) and protein level in EEECs vs. NEECs. Full-length blots/gels are presented in Supplementary Figure 1. B Western blot data of SFRP2 protein level in ectopic endometrium vs. NE C Western blot data on SFRP2 protein level. in EEECs vs. NEECs. D Real-time RT PCR. The mRNA expression levels of SFRP2 in ectopic endometrium vs Normal endometrium. E Real-time RT PCR. The mRNA expression levels of SFRP2 in EEECs vs NEECs. F MSP. The methylation rates of normal endometrium and ectopic endometrium endmetrium were detected by MSP respectively. G Bisulfite sequencing PCR on SFRP2 promoter in EEECs and NEECs.