Fig. 7From: The DNA demethylation-regulated SFRP2 dictates the progression of endometriosis via activation of the Wnt/β-catenin signaling pathwayIncrease of Wnt signaling gene expression and activity after ectopic SFRP2 expression in EEECs. A Western blot analysis. EEECs were transfected with SFRP2 cDNA or lv-control and subjected to Western blot analysis. Full-length blots/gels are presented in Supplementary Figure 1. B Western blot data of SFRP2 protein level. The protein expression levels of SFRP2 in EEECs transfected with SFRP2 cDNAvs control group. C Western blot data of beta-Catenin protein level. The protein expression levels of beta-Catenin in EEECs transfected with SFRP2 cDNAvs control group. D Real-time RT PCR. The mRNA expression levels of SFRP2 in EEECs transfected with SFRP2 cDNA vs control group. E Real-time RT PCR. The mRNA expression levels of beta-Catenin in EEECs transfected with SFRP2 cDNA vs control group. F Luciferase reporter assay. The relative TCF/LEF luciferase activity in EEECs transfected with SFRP2 cDNA vs control groupBack to article page