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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: SUMOylation of PDGF receptor α affects signaling via PLCγ and STAT3, and cell proliferation

Fig. 2

Mutation of lysine residue 917 impairs SUMOylation of PDGFRα. (a) HEK293T cells were transfected with WT or K917R mutant PDGFRα and HA-SUMO1. After serum-starvation for 24 h, cells were stimulated with PDGF-AA for 45 min, followed by immunoprecipitation with an anti-PDGFRα antibody. The proteins then were separated by SDS-PAGE and subjected to immunoblotting with indicated antibodies. The expression of PDGFRα was determined by immunoblotting of total cell lysates (TCL). The immunoblots were cropped for clarity. Full length blots are presented in Figure S1. (b) SUMOylated PDGFRα was quantified. SUMOylation levels of WT PDGFRα with stimulation of PDGF-AA at 0 min were set as 1. The experiment was performed three times and a representative blot is shown and quantified

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